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Johnny Truant posted:I don't wear a lab coat ever? even when working with gnarlies?  tbh I only wear my labcoat at the facs core because I might as well look cool while i suffer in a basement for 3 hours
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Jan 30, 2019 18:02
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Development posted:ever? even when working with gnarlies? I don't wear a white lab coat ever, nope. But if I'm doing any kind of dissection-work I'll have on a plastic gown with sleeves. That's a rad lab coat, too.
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Jan 30, 2019 18:28
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What kind of lab doesn’t require PPE? I am imagining an office of data scientists or something
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Jan 30, 2019 18:52
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Snack Bitch posted:What kind of lab doesn’t require PPE? I am imagining an office of data scientists or something Never said I don't wear PPE, in fact in the next sentence I... said I wear PPE.
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Jan 30, 2019 18:56
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Johnny Truant posted:Never said I don't wear PPE, in fact in the next sentence I... said I wear PPE. I haven’t worked anywhere that didn’t require a lab coat/glasses at all times. Seems strange, that’s why I asked
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Jan 30, 2019 19:03
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Our work either has no gloves required or two layers of gloves required, with nothing in between.
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Jan 30, 2019 19:05
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Snack Bitch posted:What kind of lab doesn’t require PPE? I am imagining an office of data scientists or something the only time i ever wear a lab coat is when we have to do stupid promotional marketing photo-shoots and videos of "HEY LOOK AT US SCIENTISTS IN LAB COATS AND SUCH" otherwise, i wear gloves when needed, same for safety glasses keep in mind i'm salty well traveled and experienced molecular biologist who doesn't give a gently caress anymore
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Jan 30, 2019 19:58
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Snack Bitch posted:What kind of lab doesn’t require PPE? I am imagining an office of data scientists or something It's amazing how bad some labs can be. One of my operators came to us because she'd quit her last job, which had her working with high-concentration sulphuric acid on open desk surfaces, no air re-circ, no PPE except for latex gloves. Ridiculous. Sundae fucked around with this message at 20:20 on Jan 30, 2019 |
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Jan 30, 2019 20:00
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Sundae posted:It's amazing how bad some labs can be. One of my operators came to us because she'd quit her last job, which had her working with high-concentration sulphuric acid on open desk surfaces, no air re-circ, no PPE except for latex gloves. That’s awful. I am very strict about safety, much to my past coworkers annoyment. Especially safety glasses, I never want to use an eye wash station.
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Jan 30, 2019 22:17
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Shrieking Muppet posted:Some how empower* found new way to be bad. While making a report I wanted it to list only vials less than 13. I tell the report to do so and low and behold I only get vials 10, 11 and 12. I look at it dumbfounded and figured ok, maybe I have to use the between function. Again I o lay get vials 10, 11 and 13. Apparently empower doesn't treat all the data as numbers and treats part as letters. Eventually found a work around but seriously what the gently caress Waters?
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Jan 30, 2019 23:15
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The most dangerous thing i use is 35% HCl, and i only use a fraction of a mL at a time.
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Jan 30, 2019 23:30
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Snack Bitch posted:That’s awful. I am very strict about safety, much to my past coworkers annoyment. Especially safety glasses, I never want to use an eye wash station. Please walk into a histology lab where they have histotechs who've been there for like 30 years, you'll have an aneurysm. They don't wear gloves  But yeah like the grizzled molecular biology cat above said, eh, I don't give a gently caress. To be fair I'm not working with incredibly hazardous stuff, and when I do I'm very careful and will put PPE on when appropriate. I have a set of work clothes that have too many bleach/haemotoxylin/eosin/silver nitrate/whatever other fun coloured chemical stains on them. Oh and I wear glasses all the time, which I know isn't a replacement for safety goggles. Actually this reminds me I should go on Zenni and see if they still have prescription goggles..
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Jan 30, 2019 23:47
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Snack Bitch posted:That’s awful. I am very strict about safety, much to my past coworkers annoyment. Especially safety glasses, I never want to use an eye wash station.
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Jan 31, 2019 01:09
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I'm pretty lax on lab coats but gloves, closed toed shoes and safety glasses I'm quite strict on for my lab. Personally I've found lab coats to be cumbersome, awkward and I've caused 3-4 spills or glassware breakages due to the lab coat alone during my in-lab career so I am pretty averse to them unless we're working with caustics or oxidants (rare for us). But yeah, safety glasses are the biggest. I got sent to urgent care because I was just walking through the lab going to my desk, not doing any work at all etc as I was just writing that day and was negligent of my PPE. I was 2-3 feet behind someone working and just happened to walk by exactly as they dropped a test tube of P. aeruginosa culture. The tube didn't break but hit the table top perfectly and launched a mL or so of culture into the air on a perfect trajectory right into my loving eye. e: it was actually kind of humorous otherwise. My friend: "did that..." me: "yeah" me: "was that" my friend: "yeah... ok lets get you to the clinic" That Works fucked around with this message at 14:51 on Jan 31, 2019 |
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Jan 31, 2019 14:47
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Lab coats are required where I work, but my position in particular doesn't really deal with anything dangerous so everything else is optional. Sometimes I wear gloves anyway just to help keep my hands from getting dry and cracked.  One time the eyewash stations were down for maintenance right when someone got a ton of hydrogen peroxide in their eyes so that was definitely An Incident that led to a lot of frantic emails and changes lol. Pikestaff fucked around with this message at 15:56 on Feb 1, 2019 |
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Feb 1, 2019 15:52
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Anyone here happen to have worked at Cambrex in the last few years?
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Feb 12, 2019 21:09
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My operators made my loving week yesterday. I received a nasty e-mail from QA wanting to know how our operators managed to tare six straight bags at exactly 140.0 grams each, when they have way higher variability than that. The record looks something like this... code:The gross weight was on the balance identified in the record, but they'd weighed the bag tare weights on a different balance which they failed to document in the record, and for which they didn't even bother making a process comment. Failure to document activities performed / correct balance. The balance they used to weigh the bags was only calibrated for down to 1KG weight. Incorrect balance, not fit-for-purpose. The balance they used to weigh the bags goes down to two decimal places in kilograms. It was reporting the bags as 0.14KG each, which the operators recorded as 140.0G. Precision? Accuracy? WHO NEEDS 'EM? They then subtracted all these things directly without accounting for significant figures. It might have worked if 140.0G was a legit measure, but noooope. Significant figures. The verifier then signed that this was all okay based on the numbers. He didn't actually see it performed. P/V duty failure. The CAPA for this investigation are gonna be so loving fun.
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Feb 12, 2019 23:42
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Holy balls. That sure sounds like an instant fire to.
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Feb 12, 2019 23:53
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Sundae posted:Balance Fun There is a reason balances always have the minimum weight marked on the cal sticker...
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Feb 13, 2019 02:37
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Brilliant co-worker ran an organic solvent through the TOC.
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Feb 13, 2019 02:46
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Mustached Demon posted:Brilliant co-worker ran an organic solvent through the TOC. For the uninformed what's a TOC?
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Feb 13, 2019 03:25
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Shrieking Muppet posted:For the uninformed what's a TOC? Total organic carbon analyzer. Usually used to see ppmish levels of organic carbon in water. Now it's full of carbon and won't return to baseline.
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Feb 13, 2019 03:47
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Total organic carbon analysis is my guess. I don't think an organic solvent will play nice with any of the TOC analysis methods. Edit what demon said
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Feb 13, 2019 03:48
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Mustached Demon posted:Total organic carbon analyzer. Usually used to see ppmish levels of organic carbon in water. Now it's full of carbon and won't return to baseline. Yeah that sounds like it's hosed
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Feb 13, 2019 10:36
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Johnny Truant posted:Please walk into a histology lab where they have histotechs who've been there for like 30 years, you'll have an aneurysm. Most of the poo poo in bio labs isn't nearly as dangerous as it's made out to be. Ethidium bromide is a great example. the only time I wear real PPE is when I'm doing a massive mouse harvest - lab coat keeps their gibs from getting on my clothing. I usually wear hospital scrubs on days I am doing big mouse harvests, too.
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Feb 13, 2019 15:39
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Edit: woops wrong thread.
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Feb 14, 2019 05:22
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Kommienzuspadt posted:Most of the poo poo in bio labs isn't nearly as dangerous as it's made out to be. Ethidium bromide is a great example.  Concentrated hydrochloric or formic acid is probably the most dangerous stuff I work with, and you bet your rear end I'm careful with those. Formic acid hurts like a motherfucker! I guess DAB is pretty bad, too?  Part of me misses doing autopsies, but I reallllllllly don't miss wearing 3 layers of PPE and sweating so much that the polymer gown becomes a second skin..
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Feb 14, 2019 13:19
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Johnny Truant posted:
Yeah I think concentrated HCl or sufuric acid are the most dangerous things I work with - if you use them in a hood with a lab coat/ it's not a problem.
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Feb 14, 2019 19:40
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I work with pyrophorics now so uh yeah, I’m not burning to death so I can be an edgy hardass in the lab
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Feb 16, 2019 12:57
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Bastard Tetris posted:I work with pyrophorics now so uh yeah, I’m not burning to death so I can be an edgy hardass in the lab Look at this non-edgy softass, everybody!  Kommienzuspadt posted:Yeah I think concentrated HCl or sufuric acid are the most dangerous things I work with - if you use them in a hood with a lab coat/ it's not a problem. Fun HCl story: we use 2M to quench AgNO3 waste after one of our stains, and for the longest time I misread the SOP and was just using concentrated to quench it; probably used 2L before I realized I just wanted so drat much, ha!
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Feb 16, 2019 13:02
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Bastard Tetris posted:I work with pyrophorics now so uh yeah, I’m not burning to death so I can be an edgy hardass in the lab My my, hey hey
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Feb 16, 2019 20:21
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Johnny Truant posted:Look at this non-edgy softass, everybody! Are you doing silver nitrate protein/carbohydrate stains? Per my protocol I quench in a methanol/acetic acid sol'n that isn't nearly as nasty as even HCl.
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Feb 17, 2019 16:48
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Kommienzuspadt posted:Are you doing silver nitrate protein/carbohydrate stains? Per my protocol I quench in a methanol/acetic acid sol'n that isn't nearly as nasty as even HCl. Modified bielschowski stain, for neuropathology and neurons/myelin  I'm still torn between formic acid and whatever weird broth they use to incubate our plasmids for worst smelling reagent. On the one hand, formic acid burns. On the other, Jesus Christ what even is this evil plasmid broth, sewer water and ammonia's bastard? 
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Feb 17, 2019 20:25
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Worst smell I think we have is tetramethylammoimium hydroxide. Smells like rotting fish and  .
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Feb 17, 2019 22:31
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lol I got a CV of .0045% today
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Feb 20, 2019 07:48
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Changed my mind, beta-mercaptoethanol is the foulest smelling thing on this goddamn planet, holy poo poo. Also, don't you love when you're in the middle of a protocol and two other people decide to piggyback halfway through said protocol without helping to prep at all, then start asking you if you did this step? Or if you did this step properly? Oh did you let this come to room temperature? ..in the middle of the protocol, when you're performing the pipetting step that requires all of your attention? And stand directly in front of the bench you're doing your work at, and not move even after you've had to navigate around them nearly six times? 
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Feb 22, 2019 18:45
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Any of y’all at pittcon this year? I’ll be in the informatics section hawking LIMS if you’re bored.
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Mar 18, 2019 16:01
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The foulest smelling substances are actually my farts
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Mar 18, 2019 17:59
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Nissin Cup Nudist posted:The foulest smelling substances are actually my farts It's an honor to meet you, E. coli.
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Mar 18, 2019 23:26
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Boss wants to buy a fluorescence spectrophotometer and we haven't used anything except a decade-old Cary Eclipse. Has anything changed recently so that there is something out there way better or cheaper and can have Peltier or other temperature control and do polarization measurements?
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Mar 19, 2019 19:35
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