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Epitope
Nov 27, 2006

Grimey Drawer

My PIN is 4826 posted:

I'm so close to dropping out. My project doesn't make any sense to me :sigh:

I have some links that might interest you-

Another student wrestling with the same question (she's stuck it out I believe)
http://forums.somethingawful.com/showthread.php?threadid=3255918

Lots of other grad students commiserating about grad school
old http://forums.somethingawful.com/showthread.php?threadid=3260704
new http://forums.somethingawful.com/showthread.php?threadid=3364373

Also my opinion- there are some for whom dropping out is a good thing, but most grad students consider it at some point and most are better off sticking it out.

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Epitope
Nov 27, 2006

Grimey Drawer

Solkanar512 posted:

This is a good point here, as I know of a guy that would do really well at a smaller firm, get bought out and join the big firm, start another small firm with guys he knew and so on and so forth. It's not like he didn't work hard, but the whole thing seemed wasteful. It was like watching skyscrapers being built only to be looted for the copper wiring.

The way I heard it described this is one way the pipeline functions. The big firms don't have enough early stage development to feed their late stage development. Someone starts a small company based on a compound or two. If things look good a big firm will buy them out, which is good since a small company probably doesn't have the resources for the late stage development anyhow.

Epitope
Nov 27, 2006

Grimey Drawer

Bastard Tetris posted:

send me your CVs if you are a technical badass with a bachelor's or a post-doc+3 yrs.

If you don't mind sharing, what sort of post doc experience do you like to see? Do you prefer postdocs from industry or academia, do you want them to know the techniques already?

Epitope
Nov 27, 2006

Grimey Drawer

Bastard Tetris posted:

Both are pretty welcome (we're partnered with UC Berkeley and UIUC), knowing the techniques beforehand will be nice but I'm not sure that it's mandatory, but with the amount of people we're interviewing I think it might as well be. Atomic Force Microscopy will be a big plus for analytical. I think we're also looking at metabolomics, synthetic biologist post-docs, and molecular bio folks.

We're also looking for scale-up and process engineers.

And I wish we could be looking for some folks in my lab, but noooooo HR wants to keep headcount down :/

Edit: That PI from the last page is a loving rear end in a top hat, I hope his patients saved by his miracle cures realize what a cocksucker he is

Good to know, thanks. I'm talking about a high-throughput screening project with a potential postdoc advisor. It is fun to talk about but I expect the work itself may be tedious- running robots and/or being a robot.

Epitope
Nov 27, 2006

Grimey Drawer

Appachai posted:

I was thinking about asking them to buy one as a part of my offer.

A new faculty here got it in his contract that he "must have access to an ultra." We joked about sabotaging the ancient departmental ones so they'd finally get some that aren't barely limping alone.

Epitope
Nov 27, 2006

Grimey Drawer

Shbobdb posted:

Check out Ed's Joblist and see if anything pops out at you.

Any chance you'd want to refer me/us?
sa.epitope@gmail.com

Epitope
Nov 27, 2006

Grimey Drawer

C-Euro posted:

Maybe getting halfway through a PhD program has given me the wrong worldview on the matter,

I was thinking this on your last post. Grad school drills into you that original research is the only thing worth a drat. Sounds like you're adapting to the different outlook in industry. Shedding the academy's worldview, finding out what's possible in industry while in the trenches, and identifying what you actually want out of life, sounds challenging.

Epitope
Nov 27, 2006

Grimey Drawer

Saros posted:

Anything specific? It's a pretty broad job.

Sorry, I should have said this out loud- We have been talking about the ethics of rushing out ebola treatments in the D&D thread. It came up whether companies were wanting to circumvent FDA processes out of a desire to do good, or out of greed. Your post stood out so I linked it in that thread.

Here's one spot where that was being discussed
http://forums.somethingawful.com/showthread.php?threadid=3661322&pagenumber=194&perpage=40#post437604835
There's a lot more in that thread, but I forget which page. Vox here is one of the main posters discussing it.

Epitope
Nov 27, 2006

Grimey Drawer
Lab Rat Movie Chat

Interstellar

Movie overall was ok I guess, a little long, but it did get my science rocks off. Specifically the "eureka!" sequence. The sequence's acting/direction maybe wasn't the best, but it still captured the eureka moment pretty well. The world (your project) depends on understanding this natural process. You even know the experiment that would get you the data you need, but it's far beyond human (grad student) capabilities. Then a lab member you had written off as lost comes through with the data in a heroic way. You crunch the numbers and see the implications. So you march into the lab where everyone is still trudging away at their work, despite being mostly resigned to the world ending (their careers contributing nothing to science). Eureka mother fuckers!

Epitope
Nov 27, 2006

Grimey Drawer

Johnny Truant posted:

Ha, that's pretty great. I've been trying to convince my lab for the ~2 years I've been here to invest in a little folding cot for when we get called in at all hours of the night, but no dice yet.

Completely unrelated, but does anyone have any experience with mold? More specifically my lab has had issues for a loooong time now about mold growing inside refrigerators we keep fixed tissue in, the boxes we keep the tissue in, the bags we keep the tissue in inside the box, and, yes, even on the tissue itself in a glycerol/DMSO cryoprotectant. We currently bleach the boxes and bags, scrub them with a brush, then give them a water rinse before going back into a cryoprotectant until we rinse again, then bag them up in fresh cryoprotectant, and we still are having issues.

I mentioned it to my supervisor the other day, but I will handle upwards of 50 brains a day in various states of freshness/fixation, and that doesn't bug me, but then I see a giant moldblob and it's like :barf:

Uhg, mold is a bitch. We got rid of one fridge because it was too infected and we couldn't keep it from re-growing.

I don't know how storing brains works, but do you need cryoprotectant if they're not being frozen? Maybe there's another storage solution that would be less prone to mold growth?

Epitope
Nov 27, 2006

Grimey Drawer

Discendo Vox posted:

To be more serious, I am actually planning to head to some division of federal research administration, so information on how to make that suck less would be helpful.

You guys like narrow RFPs, right?

Yes when they include my interest/expertise, no when they don't

Epitope
Nov 27, 2006

Grimey Drawer

Discendo Vox posted:

Tell me about the cause of this one- I'm genuinely curious.

When I've seen that it was that the entire audience was focused on research research research, and then here's this guy talking about something, and ya ya ok i get it, when is he going to get to the results, show me the data, wait its over. There was no data, nothing to grapple with, so I don't have anything to say about that. Cuz I'm just in the research mode. Also this sounds suspiciously like service, giving my time for- sure something that is important, but taking precious time from research research research. If I speak up that means I am volunteering.

Epitope
Nov 27, 2006

Grimey Drawer

DemeaninDemon posted:

That's because life itself is poo poo.

-SomethingAwful Buddha

Epitope
Nov 27, 2006

Grimey Drawer

MickeyFinn posted:

I don't know how I've only now discovered this thread.

I work for a small-ish SBIR company that specializes in technology for Department of Energy national labs for the last three years after defending and leaving grad school. I write 4-5 proposals a year and spend the other part of time working on extremely boring projects. I've gotten the feeling lately that this place is an SBIR mill as there is little to no interest in commercialization at all levels (including the DOE). But that also means there is no motivation to put much effort in to proposals because who cares, we'll get our checks do some work and in the end we'll write a report and move on the next project that is funded. Does anyone else here do SBIR contracts, is this normal?

Never applied for one yet, but when we talk about going for one the attitude seems similar.

Epitope
Nov 27, 2006

Grimey Drawer

MickeyFinn posted:

How many does you company do a year?

I'm still in the blessed academy, but I think my advisor has put in for a couple.

To share a bit more, the ideas are things that are slightly more likely to be marketable than normal academic research, so we're all "let's start a small business then we can get another grant app out," but it's still business as usual.

Epitope fucked around with this message at 10:09 on Jan 18, 2016

Epitope
Nov 27, 2006

Grimey Drawer

Ezekiel_980 posted:

Latest announcement from EHS, banning headphones in the lab soon, for reasons. I hope they ban radios as well because I could at least tolerate it then.

Good. Headphones don't belong in the lab.

Epitope
Nov 27, 2006

Grimey Drawer

goodness posted:

Are there any lab jobs I could get without a degree? Trying to get some experience for a horticulture related lab job while I'm in school for general business stuff right now.

Lab dishwasher can be a way to get in, see the inside, and meet people. For me it lead to getting a research project too.

Also, "horticulture" :crossarms: it's weed, isn't it.

Epitope
Nov 27, 2006

Grimey Drawer

Bastard Tetris posted:

Goddamn, with my network, legal transgenic weed would be way better than my current BIG PHARMA job if I didn't have to deal with all the awful blowback from the marijuana industry. Then again with dabbing they seem to have solved that problem so they can get high as gently caress without me.

What blowback is that? Anti GMOers?

Also for the record I was just poking fun. I'm definitely not anti weed, i have an application in for a license myself.

Epitope
Nov 27, 2006

Grimey Drawer

Bastard Tetris posted:

I meant more like getting straight up robbed. It happens a lot in CA dispensaries and grow operations.

Ah, yes. The marketing guy thinks we "should get shotguns," but no thank you I'm not getting in a gun fight over 4 grams of weed in methanol and a tank of helium.

Also I think I've convinced financial that all-paper is not cost effective for information/quality management. God I hope so.

Epitope
Nov 27, 2006

Grimey Drawer

I heart bacon posted:

I work at a place that makes drug test kits. Incoming drug strips and all devices that are built there are tested against human specimens along with various controls. I'm usually the first one in every day so I set up the lab and give a donation (what's the greatest nation in the world? DOnation!) It's voluntary, if that matters at all.

That seems like it might be kind of a cool data set. Do you see any fluctuation, like if you eat a poppy seed bagel?

Epitope
Nov 27, 2006

Grimey Drawer

DOOP posted:

The service manual says do not shut off the GC, our SOPs says do no shut off the GC, but

*in whiny VP voice* But what if the power goes out and there's no one here


Dude just wants to save 20 bucks on the electric bill

Restek says to plug the column if you shut down

http://blog.restek.com/?p=4495

Financial would try to save money by never changing the oil in your car

Epitope
Nov 27, 2006

Grimey Drawer
Birkenstock makes lace up full shoes, if you want to go max crunchy.

Epitope
Nov 27, 2006

Grimey Drawer
Where are you, how many samples, how much do you pay?

Epitope
Nov 27, 2006

Grimey Drawer
Sorry mate can't help ya, good luck

Epitope
Nov 27, 2006

Grimey Drawer
Putting one or two with projected submission is probably worth it, though might not count for too much. Having more projected than actual would probably look like desperately trying to pad the list

Epitope
Nov 27, 2006

Grimey Drawer

Sundae posted:

I'll take "Localized Temperature Variation" for $1000, Alex.



Calibration dudes just identified that we have a 3*C difference in temperature between the two sides of our T/%RH transmitter in one room. As-Found vs As-Left tolerance failure because last year's technician put his probe on the other side of the transmitter from this week's technician. There's some airflow fuckery going on right next to it.

I would never have thought to check that and just assumed it was a legit out-of-tolerance. :doh:

You know how if there's a cop behind you you get nervous even if you're not doing anything wrong? Cuz what if they notice something or you don't 100% stop etc. And then oops you can't find proof of insurance, now you're getting pulled into court. And anytime you got to the courthouse it's the same thing, better dress nice and not say the wrong thing, don't want to end up in jail for contempt. The justice system has a way of trying to fix every problem by applying More Justice. Which, once you're caught up in it, it can be very hard to emerge again, having a record for life etc. This results in some people (stereotypically poor) trying to avoid any contact with the justice system. Don't ever call the police, even if you need the type of help they're designed to provide. There's a similar in quality systems. The system is designed to bring things in line, and the way the system knows how to fix every problem is More Data. Until your lab is buried under a mountain of temperature probes. Maybe that's why the science poors (academics) try to avoid any type of contact with quality systems?

Epitope
Nov 27, 2006

Grimey Drawer

Dance Officer posted:

So, my boss wants to turn his business of providing chemical analysis of wines, beers and juices from a hobby gone way out of hand into a legitimate business.

I'm assuming we're gonna need to get iso 17025 accredited, but I've absolutely no experience with this.

Do you folks have experience with getting accredited, and do you have any recommendations on how to go about getting it?

I'm EU, by the way.

I've gone through something pretty similar. Are you still the only employee? Willing to hire/contract to get this done? Is the owner motivated to get the accreditation done? If he's not committed that's gonna make it pretty hard for you

I'm in the US, and far from an expert, but here's my thoughts. I'm not sure about how your accreditation body(ies) is organized. First step is to find out who is accrediting you. See if they have any education programs you could take advantage of. Once you've done some work on getting your systems in place, it might be worth getting a gap assesment. Our AB offered that, not sure if the eu one will, or if theres 3rd party auditors you could use.

Not sure how helpful my thoughts are, but happy to talk more if you want sa.epitope@gmail.com Post here if you email me, I never check that one

Epitope
Nov 27, 2006

Grimey Drawer

Shbobdb posted:

Seconding this. I recently got a job as a field service engineer (Application support specialist, field service engineer, field application specialist . . . all the same really). It is pretty awesome. I get to travel a bunch, the paycheck is sweet, I'm using my Ph.D. Check out Ed's Joblist and see if anything pops out at you.

Quoting a previous post on the topic. Get On Ed's List

Epitope
Nov 27, 2006

Grimey Drawer

C-Euro posted:

I do remember that PM and I might still have it lol. Yeah my main apprehension with this field is having to travel a bunch at a time when my wife and I are planning to have a child. However, the phone screen today made it sound like the travel area for the role is much smaller than I previously assumed and I can set my own road schedule with some flexibility (and work from home the rest of the time), which sounds much more tenable.

My mate from when we were kids' dad was an fse, and was gone all the time. They still have a good relationship, but I would say his dad being gone all the time affected him A Lot.

Epitope
Nov 27, 2006

Grimey Drawer

Cardiac posted:

We are running on both single and triple quads. Our own library is tuned by MRM, while client libraries are not.
We know the monoisotopic masses of what we inject and basically want to detect the RT for them. The RT will vary depending on column and the difference between different columns is the interesting value.

I guess I will have to go digging into how the text files are formatted to figure out a way.

Is the challenge you want to avoid manually defining RTs? Could you just make a method with RT windows spanning the whole run, export, and automate the rest from there? I only know chromatography software, so not sure if that makes sense with MS

Epitope
Nov 27, 2006

Grimey Drawer

Shrieking Muppet posted:

A member of our data review group is trying to tell me my solution preparation is wrong because a volumetric flask is less accurate than a graduated cylinder.

Day 47. Was feeling good this morning. After last night's session with Marcy in prep, I felt a break through was in the air. We were about the crack this development wide open. Then I got it, a call from DRG. Old DRG always knows how to drag you right back into the mire. I'd go for a walk in the rain to clear my head, but then I'd have to fill out an F412

Epitope
Nov 27, 2006

Grimey Drawer
That pun has a 40 year retention time

Epitope
Nov 27, 2006

Grimey Drawer
I think the ruining is setting them on the driveway, but my quality level is in the poops > 1 time per day level, so I could be mistaken

e- greater than or equal to, sorry for the confrusion, you see what i mean about my quality level

Epitope
Nov 27, 2006

Grimey Drawer
How do you know he didn't use the grasper to carefully place them on the pavement, hmmm??

The first time I saw weights in a mahogany box I thought it was bizarrely ostentatious. Now it's my go to example of how fancy can be functional. Though I'm not sure this guy would have gotten the hint.

Epitope
Nov 27, 2006

Grimey Drawer
I have a copy of Modern HPLC for Practicing Scientists, and it has a pretty general method development chapter. The table of contents is online, peep chapter 8, see if it's what you're looking for https://onlinelibrary.wiley.com/doi/pdf/10.1002/0471973106.fmatter

Epitope
Nov 27, 2006

Grimey Drawer
I knew R is big in bioinformatics, but is it big in analytical these days too? Cool. When the crusty old statistician was teaching me R is 2004 I thought for sure it was an anachronism

Epitope
Nov 27, 2006

Grimey Drawer
He has won the battle against himself. He loves big pharma

Epitope
Nov 27, 2006

Grimey Drawer

Atopian posted:

It's in a country that doesn't really care about this sort of thing, despite laws being on the books.

Sounds like my country, the United States :v: When I first delivered waste to the hazardous waste facility here, I was prepped to be good. MSDSs, labeled containers. They had me fill out paperwork, ok great. I wrote chemical names. He said oh you're not supposed to write there. He crossed them out and put "lab chemicals." On another trip they labeled them "flammable solvents" which was half the cost per pound of "lab chemicals" I've watched them decant jugs into drums with nothing but a smock and goggles, and they've asked us to change our waste stream for smell purposes. This lead me down a "should we collect in drums ourselves?" path, and lamenting their ultimate fate
https://www.theatlantic.com/technology/archive/2016/11/the-toxic-waste-drum-is-everywhere/508418/

Epitope
Nov 27, 2006

Grimey Drawer
Maybe they want to sell them on eBay and are hoping to find people who will let them go cheap?

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Epitope
Nov 27, 2006

Grimey Drawer
If the consumer doesn't care and more data gives the bureaucratic cops more ammo to hassle you with...

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