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oh boy, good tech support! Day 0: me posted:I took stack images with 900 images with Neurolucida. When I tried to save the file as a .tiff image stack, both of the files maxxed out at 2,102,674 kb, and will not open (it says they are corrupt). The program appeared to hang while I was saving it, but came back online and didn't have any popups or errors stating that the file was bad, though it did crash when I tried to do the 3d view. Day 6: quote:Dear crabrock: quote:No, both files were saved in the same computer with plenty of space, so that is not the issue. I wouldn't have been able to save another 2gb file if I was out of space for the first one. Day 8: quote:Thank you for letting us know there is plenty of room on the hard-drive. I have also forwarded the idea about the video memory to the programmers working on this and we have the file that did not save to completion. I will be away at the Society for Neuroscience meeting until the week of Thanksgiving, but the programmers will be here and hopefully make progress. If you need help in the interim, please use support.mbfbioscience.com. quote:Hi Dan, Day 9: quote:Dear Dr. Rock: (lol at getting so formal all of a sudden) Day 14: quote:Hi crabrock– Dan is at SfN and asked me to follow up internally and with you. One question that came up is: Is there sufficient disk space on the system to save your 6Gb file? i answered the question myself after some more googling. apparently a tiff can only save 2gb files. I had erroneously landed on some page saying the limit was much, much higher (but that's bigTiff). In two weeks they are still asking me the same stupid question they started with, which doesn't even make logical sense given the information i provided them. Tiff/jpg are the only formats this program will save images as. each slice in the stack is 16MB and there doesn't appear to be any way to decrease the resolution. obviously i'm not going to save high resolution microscopy photos as jpg... glad i wasted 2 weeks on this instead of just using another program to capture the images. crabrock fucked around with this message at 18:02 on Nov 14, 2017 |
# ¿ Nov 14, 2017 17:56 |
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# ¿ May 13, 2024 18:08 |
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i sent them a slightly passive aggressive response about how i just moved on to a more appropriate piece of software and told them the info i'd found and then magically within 5 minutes the responded back with quote:1. Save in .JPX format instead of .TIF. THANKS FOR YOUR FIRST RECOMMENDATION 2 WEEKS LATER
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# ¿ Nov 14, 2017 20:31 |
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hope nobody's got a grant deadline coming up :\ I did my postdoc interviews after trump got elected and it actually played a part in my decision to go to a private university instead of a public university. I mean, it was a small part, but two positions had a lot of appeal and I could have been happy at either, I think.
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# ¿ Jan 22, 2018 03:44 |
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when u do a 384 well PCR by hand but use the mouse primer instead of the rat primer
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# ¿ Feb 7, 2018 21:25 |
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my coworker dropped a whole 24 well plate of my best hippocampal sections and i replaced them and then FISH didn't work anyway
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# ¿ Feb 23, 2018 19:15 |
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anybody have any experience with xraying fixed tissue? i only ask because a friend flew with some brain sections, and now they seem to almost be repelled from our superfrost+ slides and they took like 10 times longer to mount than they should have and it was a struggle the entire time. I know you can use microwaves to fix tissue, and was wondering if an Xray would change something about them? Or it could have been the pressure of the plane... i dunno. It's just strange. Staining turned out awesome though so whatever happened to them didn't ruin them at least.
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# ¿ May 15, 2018 06:22 |
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my lab has solved this by printing a label with the password and sticking it to the monitor security theater!
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# ¿ May 20, 2018 18:15 |
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one of my labmates almost died from a faulty fume hood and the school lawyers "made" her sign a bunch of things saying she wouldn't sue the school and she signed all of them because she felt like it was her fault somehow.
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# ¿ Oct 14, 2018 20:36 |
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where are you moving?
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# ¿ Nov 29, 2018 03:29 |
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clark? i always pity the people working in there, everybody can just loving see u
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# ¿ Nov 29, 2018 03:33 |
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lol no. the power never went off in our building when that happened i guess the hospital has backups.
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# ¿ Nov 29, 2018 04:11 |
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never underestimate the importance of keeping poo poo clean/aliquoted. i simply cannot believe the amount of filth/contamination i see in labs all over the place.
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# ¿ Dec 4, 2018 18:34 |
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i left my lab notebook in the animal surgery room too long and now it smells like rat piss
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# ¿ Dec 6, 2018 20:28 |
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Artonos posted:If we're talking accidentally smelling stuff. I got a nice whiff of chloroform once. It burned the nostrils pretty bad and was a pretty small amount. a light whiff of chloroform smells kinda sweet. any more though and it's downright nasty.
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# ¿ Jan 12, 2019 05:16 |
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Does anybody know of a small glass insert i can stick in a 1.5 ml eppendorf tube? I've tried searching and can't find anything, but it seems like this has to be something somebody has wanted before... And I'm tired of cutting them myself.
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# ¿ Aug 2, 2022 19:03 |
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I don't think so. The mini slides have deparaffinized FFPE tissue sections on them. I need them to be flat for imaging and downstream stuff. The ones i posted work well, i just don't like making them myself.
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# ¿ Aug 2, 2022 22:56 |
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I'm using a diamond tip glass scorer and cutting them from regular glass slides (i want the positive charge properties). I can get 4-5 ok mini slides per slide. It's just a pain in the rear end and I'd like a lot more and not have to wash the ones I've already made. I'd probably go through hundreds (I'm in R&D and i have a lot of failures). Maybe my company will just order me some custom ones but wanted to make sure nobody already made them.
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# ¿ Aug 3, 2022 00:14 |
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Johnny Truant posted:I made the jump from academia to industry recently and jfc. This is so much better. Like infinitely better. seconded i hosed up a sequencing run and my boss was so understanding of it and we went over the thing i did wrong and then immediately planned our next one. there was no weird punishment or fretting over how much i'd just cost them or suggesting that it was because i'm stupid. just "well now you know for next time, which will be thursday" such a weird change from all the stress of messing up new assays in my previous labs
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# ¿ Aug 24, 2022 15:10 |
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i'm having some issues with an assay. about half the time it works, and the other half the time it doesn't. nobody else on my team has this issue. today another member watched me do every single step of the two day process, while doing it alongside of me. when we finished hers worked and mine didn't. we didn't figure out what i'm doing wrong, but i've successfully vexed another person who is now confused as hell
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# ¿ Sep 30, 2022 05:53 |
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my work has an airhorn taped to the wall that says "use in case of emergency" but it fell off the wall and i don't know where it went. that's ok i'm real close to the door.
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# ¿ Oct 13, 2022 03:29 |
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Douche4Sale posted:I had an undergrad once who was helping me with immunostaining. I happened to glance over and see him standing at the sink, running the tissue sections under the water and squirting hand soap on them. he's just permeabilizing for you in a cheaper way.
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# ¿ Jun 22, 2023 04:00 |
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i always figured Falcon was the NameBrand stuff made by Corning and the highest likelihood of being good, with absolutely no evidence or data to back that up. if you had problems with RNA in their stuff it's probably because you thought too hard about the RNA, and it degraded out of shame.
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# ¿ Jun 23, 2023 08:42 |
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what's the best solution for just magnetic bead cleanup. that's the part i hate the most and we have to do it 4x for our prep.
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# ¿ Aug 30, 2023 03:10 |
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i'm gud enough it's just so boring
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# ¿ Aug 30, 2023 08:30 |
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those look cool but our throughput is probably too low to justify their use. i'm in R&D and we usually are only running 2-8 samples per run, but almost every day I'm doing a library prep and it's the most monotonous and boring part of my job. do you have any knowledge about low throughput NGS library automation, like this thing? https://www.perkinelmer.com/product/bioqule-ngs-system-cls155700
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# ¿ Aug 31, 2023 09:10 |
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# ¿ May 13, 2024 18:08 |
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here, chat GPT made this for you. I helpfully had it send a slack message every time you eject a tip to notify the people who are bugging you.code:
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# ¿ May 5, 2024 20:53 |