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Dear lord I've been failing at the most simple cloning procedure for 3 weeks and now I figure out that an undergrad broke the electroporator 3 weeks ago
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Oct 5, 2021 16:17
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| # ¿ May 6, 2024 12:15 |
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Well what happened is that the undergraduate jammed in a cuvette sideways like the huge brute he is, it got stuck and the whole thing had to be taken apart and the cuvette needed to be cut out with a knife. Now the contacts seem to be misaligned but not to the point where anything is obviously wrong, apart from the fact that there's no transformants. We'll see if its repairable and in the meantime just use some other labs electroporator.
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Oct 5, 2021 17:58
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The opposite, I can literally spit into a cuvette and get normal time constants/no arcing. It's probably the contacts not touching anything.
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Oct 5, 2021 19:28
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Any good textbooks to teach myself rudimentary structural biology? I'm never going to be solving structures myself but I want to have some understanding of the colourful wiggles I keep seeing in papers. I have a PhD in microbiology so I don't need to learn bio scratch.
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Feb 9, 2022 16:08
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Crosspost from Bio thread, does anyone have an idea where I could order a bacterial strain with the classic R1 plasmid? https://en.wikipedia.org/wiki/R1_plasmid I'm asking around at my university right now but if that doesn't work out I'd rather not just cold-call the last person who published a paper on it.
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Apr 12, 2022 17:41
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I'll be starting my own (microbiology) lab soon and really want to move away from the physical lab notebooks that I've been keeping for ages. Dusting off old folders and trying to find past procedures/results from my own chickenscratch notes, let alone someone else's, is such a pain. Any experience/recommendations with that? I don't need anything fancy, ideally I'd like a situation where everyone's lab notebook is easily accessible online to me and other lab members (although that might stress out people?), and that keeps track of changes so that nobody can just go back and change their results. The simples solution would probably be google docs, but I think that's very easily editable. I've heard of people using Benchling, no idea how good it is. Any input appreciated, even just to tell me it's a horrible idea.
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May 23, 2022 19:51
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Super excited about the Alphafold -> Foldseek -> Structural Database pipeline existing now. Finally having a way to find structural homologs at blast-level speeds is amazing. I wish the ESM Metagenomic Atlas wasn't such a piece of poo poo tho with regards to telling me what I'm actually looking at...
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May 11, 2023 23:58
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Don't know about the company as a whole but I've been a big fan of Geneious Prime for 10+ years. I don't think it's an in-house development though, they purchased it some time along the way.
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Mar 7, 2024 00:55
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| # ¿ May 6, 2024 12:15 |
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Does anyone know of a good basic non-Eppendorf spectrophotometer? I've never not used an Eppendorf but they discontinued their line a while back so now it's time to get a new one and they all look janky or overpriced.
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May 5, 2024 15:20
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