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MasterBuilder
Sep 30, 2008
Oven Wrangler

University of Florida: College of Frog Killing: 104 - Frog Wrangling posted:

To humanely euthanize a Cuban treefrog, you must first capture it; there are several effective methods for doing this. The first is to simply grab the frog from a window, wall, or other perch site...Approach quickly and decisively, and with a continuous, swift movement firmly grab the frog (Figure 12). Maintain a firm grip on the frog and then simply tie the bag shut to contain the frog.

Then Freeze

University of Florida: College of Frog Killing: 202 - Icing Amphibians posted:

The easiest way to humanely euthanize a Cuban treefrog is to place the bagged frog in a refrigerator for 3–4 hours and then transfer it to a freezer for an additional 24 hours. The initial cool-down period in the fridge acts as a physical anesthetic to numb the frog so it does not feel any pain when it freezes.

Or drug them up first

University of Florida: College of Frog Killing: 401 - Fentanyl and Frogs posted:

Alternatively, you can firmly hold a recently captured Cuban treefrog and apply a benzocaine-containing ointment to the frog's back to chemically anesthetize it before placing it into a freezer to ensure death. To do this, apply a 1-inch strip of benzocaine ointment (Orajel is one popular brand) to the frog's back, rub the ointment around so it covers the back, and then after the frog has ceased to move put it in a sealed bag and place in a freezer for 24 hours. After freezing, simply remove the bagged frog from the freezer and dispose of it in the trash

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