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Appachai posted:Why not both? Well I meant that I don't know if I hate my current job function. I've been in Regulatory for two years and I still don't know whether or not I like it. But because there are some very obvious cultural problems here, I don't know if that overall dislike of my job comes from hating Regulatory work or just hating my employer specifically.
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Sep 11, 2017 05:50
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buglord posted:I mean, they called me when I was in the drive thru of a burger joint, and because my phone was paired to my car a weird way, they heard the music I was playing. But I guess that didn't scare them away either. So here's hoping something positive happens! Post the music.
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Sep 11, 2017 07:57
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Discendo Vox posted:Post the music. 
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Sep 11, 2017 19:26
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It's burgin' time! 
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https://youtu.be/gZQ5aNrwYcM phone posting so no preview. also I think I got the job 
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Sep 11, 2017 19:42
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Is it sad that this video put me over the edge on our liquid handler? We went with them, lol. https://www.youtube.com/watch?v=5RT5DofzEv0
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Sep 11, 2017 19:48
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DJ Sizzle posted:Is it sad that this video put me over the edge on our liquid handler? We went with them, lol. just lol if you don't base your choice of PCR on who won the SYBR Green vs TaqMan rap battle (it was SYBR btw)
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Sep 12, 2017 03:01
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Youth Decay posted:just lol if you don't base your choice of PCR on who won the SYBR Green vs TaqMan rap battle (it was SYBR btw) my personal favourite, featuring dr. qiadoom: https://www.youtube.com/watch?v=ISGQt9WJi5k
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Sep 12, 2017 03:21
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buglord posted:https://youtu.be/gZQ5aNrwYcM 
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Sep 12, 2017 04:06
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How much of a lab manual layout is personal preference vs a strict standard?
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Sep 12, 2017 05:38
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highly dependent on the protocol/method, or are we ralking just general wet lab guidelines?
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Sep 12, 2017 14:40
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vivisectvnv posted:highly dependent on the protocol/method, or are we ralking just general wet lab guidelines? Yeah just general guidelines. I've typically just followed the zubrick's layout if that is good enough.
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Sep 12, 2017 18:32
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Probably a long shot, but does anyone have any experience with Leica's BOND Autostaining machines?
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Sep 13, 2017 17:15
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https://twitter.com/hashtag/ScientistsTakeAKnee
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Sep 26, 2017 22:14
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Know what I'm doing when I'm done with vacationing.
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Sep 27, 2017 00:39
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white people ruin everything
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Sep 27, 2017 03:17
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I think it's fine
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Sep 27, 2017 04:27
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I am a MLT (ASCP), guessing this is the thread for me? 
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Sep 27, 2017 08:13
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So the only microbalance in our potent compound suite no longer has a backlight to it. I never thought years of playing game boy by flashlight in the back of moms car on family trips would become a skill I need at a international CRO... I did mention this to the person who oversees the balances and she said already knew and was going to contact mettler this week. How about loving right now!?
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Oct 11, 2017 19:44
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Jesus christ the two people who are supposed to be setting up our new slide scanning microscope are just sitting in chairs outside my office blabbering about hairties, after having given a "tutorial" on how to use the machine then promptly discussing between eachother all the things they don't understand about this machine with lots of "I have no idea"s and "I didn't know that at all"s interspersed in their conversation and I can still hear them through my closed door and I want to fling them into the river and
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Oct 13, 2017 20:58
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Johnny Truant posted:Jesus christ the two people who are supposed to be setting up our new slide scanning microscope are just sitting in chairs outside my office blabbering about hairties, after having given a "tutorial" on how to use the machine then promptly discussing between eachother all the things they don't understand about this machine with lots of "I have no idea"s and "I didn't know that at all"s interspersed in their conversation and I can still hear them through my closed door and I want to fling them into the river and We recently switched instruments from one company who had absolutely garbage customer service to a company with a great track record. Unfortunately this piece of equipment is new for them. During the training, it failed the test to determine if it is calibrated correctly 15 times and we were told that probably doesn't matter and the demonstrators found out that the two programs required to test and track info can't run at the same time or it crashes in the middle of testing. Our lab hears, "I've never seen that happen on this machine before" from vendors once every two months with all the new stuff we have. We were also given a prototype plate reader with brand new plates to test for them. It is the most tedious god drat thing in the world. It's self contained and you enter plate info into the reader via keyboard on a tiny led screen. It drops inputs if you type too quickly. We run hundreds of plates a day and there's no way we could read that amount in a day with this thing. My boss won't stop praising this thing while just kind of ignoring that no one has used it since the first time we used it.
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Oct 13, 2017 23:27
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Phosphene posted:We recently switched instruments from one company who had absolutely garbage customer service to a company with a great track record. Unfortunately this piece of equipment is new for them. During the training, it failed the test to determine if it is calibrated correctly 15 times and we were told that probably doesn't matter and the demonstrators found out that the two programs required to test and track info can't run at the same time or it crashes in the middle of testing. Our lab hears, "I've never seen that happen on this machine before" from vendors once every two months with all the new stuff we have. I've got a training session on the microscope next week, with one of the same techs that was a part of the initial clusterfuck. Oh boy! Also I've been running so many titrations that I think I'm going to start seeing them in my sleep 
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Oct 19, 2017 16:18
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So glad I came in on a Sunday to do some immunos, and nothing was visualized with our DAB in our autostainer. I love wasting three hours of my time because of a piece of trash machine!
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Nov 5, 2017 21:16
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That's like 80% of all lab work: instrument not working right.
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Nov 5, 2017 21:40
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Mustached Demon posted:That's like 80% of all lab work: instrument not working right. Would I rather do immunos by hand, or be pissed off at machinery... 
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Nov 5, 2017 23:46
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Mustached Demon posted:That's like 80% of all lab work: instrument not working right. We restarted a pc today and no one we could reach knew the password to get back into the computer so we couldn't use that instrument today. How does this happen.
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Nov 6, 2017 00:48
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Mustached Demon posted:That's like 80% of all lab work: instrument not working right. gently caress Tecan Evo robots, seriously. Supposed to let us run more assays more accurately but they mostly create very expensive headaches.
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Nov 7, 2017 04:40
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Youth Decay posted:gently caress Tecan Evo robots, seriously. Supposed to let us run more assays more accurately but they mostly create very expensive headaches. Like what? Ours works rather well, but is mostly used for pipetting crystallization screens and DSF plates.
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Nov 7, 2017 17:58
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Cardiac posted:Like what? Most recent one was a tiiiny error in a script that caused it to gently caress up when pipetting a partial plate for a nAb titer, which somehow took 6 weeks to fix and revalidate the assay. In the meantime we've accumulated a backlog of ~800 samples, can run only 3 per plate and the script can only do 4 plates at a time, and we'll be able to use at most 2 Tecans a day - they're expecting the first data transfer in early December. Doing the math, that ain't gonna happen.
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Nov 12, 2017 04:02
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I love when someone requesting tissue from my lab asks for an absurd amount of a region of interest, then when told that is not feasible, switches to another region of interest, also an absurd amount.  People in academia can be so thoughtless(myself surely included).
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Nov 14, 2017 15:10
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What do you mean you can't get me 4 kilograms of sectioned murine occipital lobe from immunosuppressed mice expressing gene (whatever)? HOW WILL I DO MY WORK? Minus the kilograms part, I occasionally see R&D distribution-list e-mails go around that are like that. "Hey, anyone have a spare set of plated spleen cells from a parrot, ideally coated with a fine layer of ear wax? It's for research of course." Come on already. You know nobody has that, and if they do, they sure as gently caress aren't giving it to you. Sundae fucked around with this message at 17:39 on Nov 14, 2017 |
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Nov 14, 2017 17:37
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oh boy, good tech support! Day 0: me posted:I took stack images with 900 images with Neurolucida. When I tried to save the file as a .tiff image stack, both of the files maxxed out at 2,102,674 kb, and will not open (it says they are corrupt). The program appeared to hang while I was saving it, but came back online and didn't have any popups or errors stating that the file was bad, though it did crash when I tried to do the 3d view. Day 6: quote:Dear crabrock: quote:No, both files were saved in the same computer with plenty of space, so that is not the issue. I wouldn't have been able to save another 2gb file if I was out of space for the first one. Day 8: quote:Thank you for letting us know there is plenty of room on the hard-drive. I have also forwarded the idea about the video memory to the programmers working on this and we have the file that did not save to completion. I will be away at the Society for Neuroscience meeting until the week of Thanksgiving, but the programmers will be here and hopefully make progress. If you need help in the interim, please use support.mbfbioscience.com. quote:Hi Dan, Day 9: quote:Dear Dr. Rock: (lol at getting so formal all of a sudden) Day 14: quote:Hi crabrock– Dan is at SfN and asked me to follow up internally and with you. One question that came up is: Is there sufficient disk space on the system to save your 6Gb file?  i answered the question myself after some more googling. apparently a tiff can only save 2gb files. I had erroneously landed on some page saying the limit was much, much higher (but that's bigTiff). In two weeks they are still asking me the same stupid question they started with, which doesn't even make logical sense given the information i provided them. Tiff/jpg are the only formats this program will save images as. each slice in the stack is 16MB and there doesn't appear to be any way to decrease the resolution. obviously i'm not going to save high resolution microscopy photos as jpg... glad i wasted 2 weeks on this instead of just using another program to capture the images. crabrock fucked around with this message at 18:02 on Nov 14, 2017 |
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Nov 14, 2017 17:56
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crabrock posted:oh boy, good tech support! This is making me really look forward to using our new slide-scanner and image analysis program! Especially because the trainers for it seemed to have no idea what they were doing..
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Nov 14, 2017 18:08
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crabrock posted:oh boy, good tech support! I'm laughing maniacally because I had a similar convo with Olympus over some confocal software literally a decade ago that was about the same as this.
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Nov 14, 2017 18:12
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i sent them a slightly passive aggressive response about how i just moved on to a more appropriate piece of software and told them the info i'd found and then magically within 5 minutes the responded back with quote:1. Save in .JPX format instead of .TIF. THANKS FOR YOUR FIRST RECOMMENDATION 2 WEEKS LATER
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Nov 14, 2017 20:31
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Sundae posted:What do you mean you can't get me 4 kilograms of sectioned murine occipital lobe from immunosuppressed mice expressing gene (whatever)? HOW WILL I DO MY WORK? I just keep imagining this request being made at the counter of a deli. "Hey, can I get a pound of the pressed murine substantia nigra loaf? The DBA/2J? And microtome it thin? No, thinner than that."
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Nov 14, 2017 20:41
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That Works posted:I'm laughing maniacally because I had a similar convo with Olympus over some confocal software literally a decade ago that was about the same as this. lovely software seems common in labs. Our ICP-oes politely forgets to calculate out dilution factors when printing reports. So the printed reports don't have dilution corrected values. They said they'd get the error like a year ago. Thankfully we can copy and paste the data into a spreadsheet...
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Nov 14, 2017 21:41
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Mustached Demon posted:lovely software seems common in labs. I don't think i've even found lab software that didn't have glaring deficiencies. when you add in GMP compliance a desire to drink the medical grade ethanol becomes understandable. my favorite is that bruker NMR software in some versions the audit trails don't even record integration.
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Nov 15, 2017 00:35
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Sundae posted:What do you mean you can't get me 4 kilograms of sectioned murine occipital lobe from immunosuppressed mice expressing gene (whatever)? HOW WILL I DO MY WORK? Just to come back to this, it happened TWICE to me today. "One gram of substantia nigra minimum, from ten PD cases and ten controls" motherfucker there's maybe a gram of substantia nigra TOTAL, and gently caress you for then trying to sneak in getting you 20g from the prefrontal cortex without following our tissue request protocol. Have fun resubmitting that form and getting bumped to the bottom of our distribution que  "We need 100g of frontal cortex from ten controls and ten AD patients so we can try to narrow down the heterogeneity of tau" MOTHERFUCKER THAT'S THE WHOLE CORTEX. HOW CAN YOU ASK FOR LITERALLY TWENTY OF OUR BRAINS FRONTAL LOBES I did not realize how frustrating being a tissue bank coordinator would be
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Nov 15, 2017 03:21
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you sure they didnt mean 100g en masse? as in 10g per sample?
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Nov 15, 2017 05:04
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vivisectvnv posted:you sure they didnt mean 100g en masse? as in 10g per sample? ... Yes, I'm sure. If it was total mass I don't think I'd be posting about it here.
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Nov 15, 2017 16:20
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