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Nov 21, 2019 00:46
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| # ? May 31, 2024 18:16 |
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norton I posted:A fun ad I found in some lab supplies. Is this for injecting the brain after being removed? Because isn’t that what stereotactic surgery is for?
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Nov 21, 2019 00:50
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Fister Roboto posted:jesus christ why I forget which vaccine it was, but they used to use mice to incubate a certain pathogen. Then they blended the mice and extracted the vaccine. Something like that. I don't think they do it anymore at least.
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Nov 21, 2019 00:50
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KHLAV KALASHNIKOV posted:Is this for injecting the brain after being removed? Because isn’t that what stereotactic surgery is for? These syringes are used for stereotactic injections
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Nov 21, 2019 00:55
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norton I posted:It's for mice, which leads to the classic mixer ad: I used this for RNA tissue extraction from mouse and rat organs in my graduate degree like a million times. It’s a good product I fully endorse it.
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Nov 21, 2019 01:19
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"Karen, I'm in pain. Could you please no- Please delete that. No, you're not posting it on Facebook. No! You're not sending it to your sister! Damnit, Karen! This is why Emily's staying at school for Christmas again!"
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Nov 21, 2019 01:34
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Blind Rasputin posted:I used this for RNA tissue extraction from mouse and rat organs in my graduate degree like a million times. What else did you put in there? Don't lie to us...
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Nov 21, 2019 01:39
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Blind Rasputin posted:I used this for RNA tissue extraction from mouse and rat organs in my graduate degree like a million times. How much foaming did you encounter though?
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Nov 21, 2019 01:44
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Nov 21, 2019 02:35
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If you want a maritime traditional non-sinking tattoo you get a pig and rooster 
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Nov 21, 2019 02:41
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canyoneer posted:If you want a maritime traditional non-sinking tattoo you get a pig and rooster 
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Nov 21, 2019 02:44
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Friend posted:yeah she said the city came by, I assume they "resolved" the rooster's head from its body Coq au vin
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Nov 21, 2019 03:06
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Fister Roboto posted:jesus christ why Because waiting a full minute for a soup-like homogenate of mouse is bullshit
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Nov 21, 2019 03:35
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Soup-like Homogenate is a pretty pro username.
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Nov 21, 2019 03:40
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"Remove to sink"
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Nov 21, 2019 03:50
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it’s such a pain when your have to wipe the side of your nose with your finger and stir your homogenate to get the foam to go down
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Nov 21, 2019 03:54
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Betrayed by his disciple, sacrificed himself, and came back from the dead. Close enough.
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Nov 21, 2019 04:43
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Really, it's your own fault for assuming your pokemon owes you emotional labor.
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Nov 21, 2019 04:50
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MizPiz posted:Really, it's your own fault for assuming your pokemon owes you emotional labor. Even if it's a Reshiram? https://youtu.be/lPyhlybViJw
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Nov 21, 2019 05:04
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Blind Rasputin posted:I used this for RNA tissue extraction from mouse and rat organs in my graduate degree like a million times. Did you put the entire mouse in there, or dissect out the organs first?
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Nov 21, 2019 05:06
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How does it.. reduce the mouse? 27k RPMs sounds like a lot, does it just liquify?
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Nov 21, 2019 05:17
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Admiral Joeslop posted:How does it.. reduce the mouse? 27k RPMs sounds like a lot, does it just liquify? Mice (and humans, and most other animals for that matter) are mostly water. Smash up the solid bits into small enough pieces and what's left is soup.
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Nov 21, 2019 05:20
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Or a smoothie, if you add some ice cubes before you grind.
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Nov 21, 2019 05:22
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Mmm mouse soup, just like momma used to make.
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Nov 21, 2019 05:22
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You have to strain it through a coffee filter if you're going to use it in a paint sprayer though
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Nov 21, 2019 05:23
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Admiral Joeslop posted:How does it.. reduce the mouse? 27k RPMs sounds like a lot, does it just liquify? Well, it's not simmering on a low heat for 20 mins, that's for sure.
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Nov 21, 2019 05:23
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So it's a tiny super grinder but for mice. Gross but also rad.
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Nov 21, 2019 05:24
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Deteriorata posted:Mice (and humans, and most other animals for that matter) are mostly water. Smash up the solid bits into small enough pieces and what's left is soup. The impressive part isn't turning it into soup, it's the fact that it doesn't aerate it.
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Nov 21, 2019 05:27
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Inceltown posted:The impressive part isn't turning it into soup, it's the fact that it doesn't aerate it. *minimizes aeration. You know the saying: you can't reduce an entire mouse to a soup-like homogenate in 30 seconds without creating a little foam.
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Nov 21, 2019 05:50
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Admiral Joeslop posted:So it's a tiny super grinder but for mice. Gross but also rad. A grinder just gets you meat paste. A homogeniser specifically disrupts all the individual cells so you can get at the cytoplasm.
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Nov 21, 2019 06:11
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The Lone Badger posted:Did you put the entire mouse in there, or dissect out the organs first? Dissect out the organs, maybe a liver or in my case part of the brain. Flash freeze on liquid nitrogen. Then put the piece of tissue in a tube containing RNA extraction solution. It’s really just phenol/chloroform and some enzymes. Use that tissue blender to totally homogenize the tissue and cavitation breaks all the cells open releasing the DNA and RNA. It doesn’t foam up very much at all, and works amazingly well, actually. Then you use a high speed centrifuge to separate the organic and aqueous phase, get the DNA/RNA by pipeting out the aqueous phase into a fresh tube. Clean the FNA/RNA with a few mixes of ethanol/isopropyl alcohol to clean away any proteins or phenol that may still linger, then precipitate the DNA/RNA into a pellet using ethanol and high speed centrifuge. DNA/RNA aren’t soluble in ethanol so it turns into a pellet at the bottom of the tube under 13,000g. Then, submit the super pure genetic material to a DNAse reaction to destroy all the DNA and leave just RNA. Then, after all that, you can run quantitative PCR on the RNA to determine just how active your favorite certain genes were at the moment you lopped that mouses head off and cut out it’s brain! As an aside, phenol is super weird tasting and smelling and it’s what is in chloroseptic throat spray, so every time I use that stuff I think of mouse brains. Blind Rasputin has a new favorite as of 06:19 on Nov 21, 2019 |
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Nov 21, 2019 06:16
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I wish I could find this ad I had for those mouse grinders because it had advertising statements like "Have you ever had difficulty homogenizing a mouse within 30 seconds?"
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Nov 21, 2019 06:22
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i'm bout to reduce this mans entire career into a soup-like homogenate
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Nov 21, 2019 06:22
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The Lone Badger posted:A grinder just gets you meat paste. A homogeniser specifically disrupts all the individual cells so you can get at the cytoplasm. We all know that grindr gets you meat paste but cytoplasm doesn't sound sexy at all.
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Nov 21, 2019 06:27
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Biological/medical research is pretty gross at times, but when you do it day in and day out it gets mundane as hell. It's around then that you look at add like that and think, yes, I hate it when there's excessive foaming in my homogenate (foaming is really annoying btw) and this thing looks like it might solve that problem. Just all me how quickly I can kill a mouse, extract its spleen, thymus, and four of its major lymph nodes. I had to do it several hundred times for one of my research projects and I got quite good at it.
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Nov 21, 2019 06:46
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We also broke down the organs after we extracted them, but we did that by hand using a pair of microscope slides since we still wanted intact cells and not just lysates.
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Nov 21, 2019 06:50
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Mice are a profoundly important backbone of biomedical research throughout the world. They are 87% genetically identical to humans, the structure of their genome all the way down to epigenetic methylation and microRNA behaviors is identical to ours, and their organs and even microstructure of their brain and many other organs (I’d say the only exception here is their kidney is a single large nephron unlike ours) is identical to ours. Also, it only takes 19 days to make a whole bunch of them. They get pregnant the same day they deliver, and birth up to 12 pups a litter. Every 19 days. The advanced tools we have also allow us to precisely manipulate nearly any gene in their genome and make mutated mice within weeks to months to study things in science. So it’s super important to give them a respectful and humane death. It’s actually vitally important in endocrinology and neuroendocrinology that the mouse not suffer before it’s death, not only for itself, but since this can radically change which genes are turned on or off in the panic state. The way they are put down before an experiment is highly regulated. I have never seen a machine or that homogenizer used on a whole animal. I don’t think it would work as advertised. I just like.. don’t see it happening without a huge mess on your hands. It’s amazing for frozen tissue samples though. As an aside, like above mice breed exceedingly fast. What’s 12x every 19 days equal in a year if they can mate with their brothers and sisters after a month? Basically what I’m trying to say is, if you ever see a single mouse in your house, there’s probably 20 more somewhere in the walls or crawl space.
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Nov 21, 2019 06:52
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Prof. Banks posted:Biological/medical research is pretty gross at times, but when you do it day in and day out it gets mundane as hell. It's around then that you look at add like that and think, yes, I hate it when there's excessive foaming in my homogenate (foaming is really annoying btw) and this thing looks like it might solve that problem. Just all me how quickly I can kill a mouse, extract its spleen, thymus, and four of its major lymph nodes. I had to do it several hundred times for one of my research projects and I got quite good at it. This post is why Fritz Haber's whole family killed themselves btw
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Nov 21, 2019 06:52
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Blind Rasputin posted:Mice are a profoundly important backbone of biomedical research throughout the world. They are 87% genetically identical to humans, the structure of their genome all the way down to epigenetic methylation and microRNA behaviors is identical to ours, and their organs and even microstructure of their brain and many other organs (I’d say the only exception here is their kidney is a single large nephron unlike ours) is identical to ours. Also, it only takes 19 days to make a whole bunch of them. They get pregnant the same day they deliver, and birth up to 12 pups a litter. Every 19 days. The advanced tools we have also allow us to precisely manipulate nearly any gene in their genome and make mutated mice within weeks to months to study things in science. You have the worst pillow talk ever.
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Nov 21, 2019 06:54
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Nice username/post combo there.
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Nov 21, 2019 06:55
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