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Dik Hz posted:Speaking as someone who had a forced IT update brick a mass spec, the techs are correct. If a computer is hooked up to a mass spec, it should not be connected to a network. All antivirus and firewall should be nuked. Customer wants to acquire 600 MB data files and send them to amazon web services over an internet connection instead of just ANALYZING THE DATA ON THE MOTHERFUCKING ACQUISTIION PC LIKE A NORMAL PERSON. They have 0 tolerance for interruptions oh also they want to lock the PC down with all sorts of domain security restrictions since a computer is just a computer if it works for sales it will work for a data acquisition pc. "Hi,, hello, I tried to load 50 blue ray movie loving data files and it took a few minutes to open--I'm furious please solve this"
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Aug 6, 2021 01:57
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Epitope posted:Y'all mean no network with internet right? Or are you carrying data on a thumb drive or something? So, yeah. IT hates it, but I hate them bricking my GC-MSs even more.
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Aug 6, 2021 02:43
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I would love to get these rickety instruments off the network. Lab managers love being able to remote into machines (especially since Covid hit) and put data on file shares. I lose.
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Aug 6, 2021 03:56
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We might Intranet the mass spec machines. There’s a piece of software called Specbase promising to automatically extract performance data. If it works, we would not have to put QC levels and back pressure manually into a billion spreadsheets.
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Aug 6, 2021 08:08
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Needs to be a way to keep the instrument PC off the network, but with a second networked PC that can be control/Waldo remote into it. Only mouse/keyboard in, only data/screen out.
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Aug 6, 2021 17:19
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Oh joy, another headache, seems the HCl salt I’m testing is missing about 10% of the Cl…
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Aug 6, 2021 19:41
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street doc posted:Needs to be a way to keep the instrument PC off the network, but with a second networked PC that can be control/Waldo remote into it. Only mouse/keyboard in, only data/screen out. I've got a meeting on Monday about that !
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Aug 7, 2021 06:11
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mycomancy posted:Lol lmao I build my own HTS pipelines using python and command-line software. Geneious and Benchling are mostly relegated to annotation visualization and primer design/storage. my man also you don’t work with a bunch of protein scientists on the edge of retirement that don’t give a fuuuuuuck about any of this
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Aug 9, 2021 06:07
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I run an academic research lab so I make the students build pipelines usually from some mixture of R, python and shell scripts and make sure it all runs on our university Linux cluster. It's more painful up front but they are a ton better at doing stuff after going through it instead of using CLC or Geneious. Plus we can spin up an AWS instance and run everything if (when) the school decides to shut down research computing.
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Aug 9, 2021 21:13
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Shrieking Muppet posted:Oh joy, another headache, seems the HCl salt I’m testing is missing about 10% of the Cl… Instrument failure or extremely exotic situation?
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Aug 10, 2021 07:10
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Atopian posted:Instrument failure or extremely exotic situation? Seems to be a problem making the compound, apparently its failed 3 other tests as well.
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Aug 10, 2021 11:12
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Bastard Tetris posted:my man Ugh, old biochemists are some of the crankiest of all the life scientists. I think only old female ecologists are crankier, though they usually have much better reasons to be cranky.
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Aug 10, 2021 21:01
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mycomancy posted:Ugh, old biochemists are some of the crankiest of all the life scientists. I think only old female ecologists are crankier, though they usually have much better reasons to be cranky. all my old academic advisors are grey haired (or bald from chemo) cranky ecologist women so I am at peace walking among them
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Aug 10, 2021 21:39
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Old microbiologists, at least the ones who aren't sexpests, are generally high functioning and cheerful alcoholics. Good company usually.
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Aug 11, 2021 02:17
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That Works posted:Old microbiologists, at least the ones who aren't sexpests, are generally high functioning and cheerful alcoholics. Can confirm
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Aug 12, 2021 09:23
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That Works posted:Old microbiologists, at least the ones who aren't sexpests, are generally high functioning and cheerful alcoholics. I feel seen
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Aug 12, 2021 13:40
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Anyone have experience with a tremor disorder and pipettes? I'm moving to a new lab soon, and they've got a pot of money for disability accommodations. I've heard about Ovation pipettes, but I'd prefer something that doesn't need custom tips.
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Aug 20, 2021 11:37
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AfricanBootyShine posted:Anyone have experience with a tremor disorder and pipettes? I'm moving to a new lab soon, and they've got a pot of money for disability accommodations. I've heard about Ovation pipettes, but I'd prefer something that doesn't need custom tips. Check out Viaflow Integras, or get an Andrew+ and drive your whole workflow with Onelab. https://www.andrewalliance.com/pipetting-robot/
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Aug 22, 2021 10:20
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The integra is both saving us tons of time on 96-well plate Vit D preps, and also a massive pain in the rear end. Aparently you have to program it using that little iPod they stapled to it as a user interface. Also we need to do a solvent exchange on our spicier preps so we don't melt their internal o-rings.
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Aug 22, 2021 17:32
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Lunar Suite posted:The integra is both saving us tons of time on 96-well plate Vit D preps, and also a massive pain in the rear end. Aparently you have to program it using that little iPod they stapled to it as a user interface. If you go on the Integra site they have free software that allows you to write methods, store them as XML, and push them to the pipettes with their com cables. They also have an assist robot you can attach the pipettes to as well. Also see if your rep can swap the o-rings out with PEEK or something less vulnerable to spicy prep attacks.
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Sep 25, 2021 23:01
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Dear lord I've been failing at the most simple cloning procedure for 3 weeks and now I figure out that an undergrad broke the electroporator 3 weeks ago
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Oct 5, 2021 16:17
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married but discreet posted:Dear lord I've been failing at the most simple cloning procedure for 3 weeks and now I figure out that an undergrad broke the electroporator 3 weeks ago Break the undergrad.
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Oct 5, 2021 16:33
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street doc posted:Break the undergrad. Or do cold shocks (not on the undergrad) with calcium competent cells. I have horrible memories of an electroporator.
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Oct 5, 2021 16:39
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Cardiac posted:Or do cold shocks (not on the undergrad) with calcium competent cells. Chem comp cells are a pain in the rear end for anything other than Gibson cloning. As for the electroporator, how is it broken? I've actually repaired one before that simply had a bent electrical contact. Once I straightened it out it worked great.
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Oct 5, 2021 17:48
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mycomancy posted:Chem comp cells are a pain in the rear end for anything other than Gibson cloning. I say this as I literally am walking to lab to dunk cold cells into warm water like a loving caveman.
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Oct 5, 2021 17:49
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Well what happened is that the undergraduate jammed in a cuvette sideways like the huge brute he is, it got stuck and the whole thing had to be taken apart and the cuvette needed to be cut out with a knife. Now the contacts seem to be misaligned but not to the point where anything is obviously wrong, apart from the fact that there's no transformants. We'll see if its repairable and in the meantime just use some other labs electroporator.
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Oct 5, 2021 17:58
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married but discreet posted:Well what happened is that the undergraduate jammed in a cuvette sideways like the huge brute he is, it got stuck and the whole thing had to be taken apart and the cuvette needed to be cut out with a knife. Now the contacts seem to be misaligned but not to the point where anything is obviously wrong, apart from the fact that there's no transformants. We'll see if its repairable and in the meantime just use some other labs electroporator. Are you getting strange time constants from empty cassettes or cassettes filled with millique water?
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Oct 5, 2021 18:18
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The opposite, I can literally spit into a cuvette and get normal time constants/no arcing. It's probably the contacts not touching anything.
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Oct 5, 2021 19:28
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mycomancy posted:I say this as I literally am walking to lab to dunk cold cells into warm water like a
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Oct 5, 2021 21:48
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Bastard Tetris posted:Check out Viaflow Integras, or get an Andrew+ and drive your whole workflow with Onelab. Andrew+ is good stuff, although I had to train a couple of my team up on Linux support to end a 6 month struggle to get the thing set up.
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Oct 5, 2021 21:58
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married but discreet posted:The opposite, I can literally spit into a cuvette and get normal time constants/no arcing. It's probably the contacts not touching anything. Yeah the copper clamp inside is bent. Open it up, bend it back into place, and I bet it works fine. My clamp was bent the other way, was sparking on an empty cuvette.
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Oct 6, 2021 01:00
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If anyone wants to work for a LIMS vendor we are always looking for Business Analysts, positions would be remote.
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Oct 8, 2021 17:06
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Lyon posted:If anyone wants to work for a LIMS vendor we are always looking for Business Analysts, positions would be remote. When I daydream about quitting my job I want to think about living on a sailboat, not working for a different piece of the money machine  (for real though that sounds kinda intriguing)
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Oct 8, 2021 18:59
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If you're tired of lab bullshit I highly recommend going the lims route! And from my job hunt this past summer can confirm Lyon's company has a lot of market share and direct experience with their software is highly desirable. I jumped from lab to lims in Jan 2020 and it was a fantastic choice!* *must be able to handle large amounts of regulatoryly required documentation / validation work.
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Oct 8, 2021 19:51
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Spikes32 posted:If you're tired of lab bullshit I highly recommend going the lims route! And from my job hunt this past summer can confirm Lyon's company has a lot of market share and direct experience with their software is highly desirable. I jumped from lab to lims in Jan 2020 and it was a fantastic choice!* Yeah we may not be number one yet but we will be soon. And the spoiler is very much accurate, business analyst roles are primarily documentation (user requirements, functional requirements, validation, etc.) and customer meetings. The travel is relatively light generally speaking but there’s always a chance for it to jump depending on the project(s) you’re assigned to. Salary probably ranges from $80k - $120k DOE.
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Oct 8, 2021 20:32
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Lyon posted:Yeah we may not be number one yet but we will be soon. And the spoiler is very much accurate, business analyst roles are primarily documentation (user requirements, functional requirements, validation, etc.) and customer meetings. The travel is relatively light generally speaking but there’s always a chance for it to jump depending on the project(s) you’re assigned to. I know an MBA in Boston that would be interested. PM me details.
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Oct 8, 2021 22:57
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I was the primary managing our LIMS deployment on a shoestring budget at my company after the analytical lab lead selected a vendor and then bounced because she was awful. Then we had to pick up and move the LIMS from a local box to AWS and again had no money to do it. So that’s how me, a chemist, learned to configure LabWare lims on an AWS environment. That program has some janky configuration design choices.  Still better than the excel spreadsheets and VBA code we were using to manage samples and results.
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Oct 15, 2021 15:24
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You got labware running on aws by yourself? Daaaamn I'm impressed. Labware is a hot steaming pile of very old code design. But for a LIMS system from a functional viewpoint it can be extremely effective.
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Oct 15, 2021 16:08
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The DB structure itself isn’t terrible. There are a ton of tables we basically don’t use, but it’s super easy to write queries to get data. The docs for LabWare are just awful. I have a lot of experience in python and some in VBA, so LIMS basic was pretty easy to figure out. The nightmare was figuring out how to configure the weblims to work. LabWare gives no guidance for how to configure something like tomcat to serve it.
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Oct 15, 2021 16:33
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Yeah, there's a lot that works with the database and lims basic. But they want you to use their consultants for anything too complicated especially weblims which is still relatively new. Are you still doing this, and if so do you have access to the support boards / list serve? I got a lot of help that way
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Oct 15, 2021 16:42
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