(Thread IKs:
PoundSand)
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euphronius posted:that’s when flu season starts it’s good there are no big social events that take place on the final day of October
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Sep 21, 2023 00:50
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Going to ask every gov official how many tests one test is
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Sep 21, 2023 00:50
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yoloer420 posted:The primer I was looking at makes it very easy to just eyeball the result. Yellow = covid, red = no covid. In most lab settings, you're running PCR (or LAMP) in an instrument that both controls temperature as needed and takes images of the reactions after each cycle or (in LAMP's case) time points. There are dyes in the reaction that, if the reaction is positive and DNA is getting copied, absorbs one color of light and emits another (this is called fluorescence). Most commonly, it's blue absorbance and green emission, but there are a ton of options, and you can run a few in the same reaction as long as the colors are different enough and detect multiple targets at once. So generally you're tracking whether you're getting signal from the dye that says "positive." If it increases enough, the reaction is positive. If it doesn't, it's negative. There are a lot of kinds of dyes; if you really want to nerd out, this is a good breakdown: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/qpcr/quantitative-pcr-and-digital-pcr-detection-methods You can also use other methods like electrochemical crap, or straight-up opening the reaction when it's done, looking at the sizes of the DNA in it, and determining whether the expected size of your target is there. (That is, you would know that your reaction is making, say, an 80-base pair product, so if you see a nice 80-bp band in your analyzer then the reaction happened.) Hell, LAMP in particular makes so drat much DNA, it turns the reaction cloudy, so you can just look for the cloudiness in a pinch.
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Sep 21, 2023 00:57
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visible concentrations of DNA is a LOT lol
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Sep 21, 2023 01:12
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Bastard Tetris posted:visible concentrations of DNA is a LOT lol
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Sep 21, 2023 01:13
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Incidentally, LAMP is especially prone to carryover contamination
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Sep 21, 2023 01:15
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Vaxxed? https://twitter.com/pitchfork/status/1704513640236196118 (USER WAS PUT ON PROBATION FOR THIS POST)
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Sep 21, 2023 01:16
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Phlag posted:Walgreens does offer PCR tests through a partnership with DOH. I'm not saying that to disagree or prove you wrong - just hoping that some unaware folks are able to snag them. I totally agree that high quality tests should be free and far more easily accessible. None of the Walgreens within an hour drive of me have had them in stock in weeks (at least when I've checked). Wow, there is actually a walgreens doing this near me. Last I checked in on this a few months ago there wasn't anything within like a hundred miles
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Sep 21, 2023 01:17
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Zugzwang posted:Ah ok, that's LAMP, not PCR, though they are related chemistries (both exponentially copy the target genetic region). LAMP is indeed much easier in a DIY setting because it only needs one temperature, whereas PCR requires cycling multiple temperatures. Thanks for the detailed reply! That helps me a lot, understanding that the difference between PCR and LAMP is the single temperature in the process is helpful. Also that the PCR instrument takes images, that explains a bit to me, most of the explanations I found were just about precise timing/temp control, which is really missing that key part of the explanation. I'll take a look at that link, thanks! Are there issues with PCR vs LAMP for covid testing? Is one more precise than the other? or are the reagents cheaper for PCR? I'm just wondering why PCR is used when LAMP seems easier in every possible way.
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Sep 21, 2023 01:19
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please don't do this, it's (a) weird, and (b) no need to guess since it was probably loving covid, and thus redundant. Anyway, lmao if covid just ends making a streak of one term presidencies through end of term hellwaves. (USER WAS PUT ON PROBATION FOR THIS POST)
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Sep 21, 2023 01:21
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Isn't this what Bieber is dealing with for the last couple of years?
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Sep 21, 2023 01:22
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Bixington posted:I've encountered one healthcare worker masking at my dentist, but ones at cancer, allergy, etc. are completely absent. Like...the usually old people at these places are incredibly at risk...wtf. Said it before ITT, height of Delta, visiting family in hospice. Just a nonstop stream of unmasked healthcare workers/family/patients walking into the Dialysis clinic next door with nary a mask in sight.
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Sep 21, 2023 01:22
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again, no need to speculate, we all know what was likely responsible. it's only interesting if they break omerta, as more are doing.
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Sep 21, 2023 01:23
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Respiratory Illness Dashboard. I'm presenting recent cases, emergency room (ER) visits, hospitalizations, and deaths for Washington state highlighting changes in reporting due to backfill. These are cobbled together from reports published by Washington's Healthcare, Emergency, and Logistics Tracking Hub (WA HEALTH), Washington Disease Reporting System (WDRS), Washington Health and Life Event System (WHALES), CDC's Weekly Deaths, and HHS' Hospital Capacity by State and Hospital Capacity by Facility. Thankfully I found everything reporting a standardized Sunday-Saturday week, the only downside is that the reports themselves update with different dates and frequencies. Details are in each item's description below. This is my first week reporting with the new setup so bear with me as I get the kinks sorted out. Full set of images linked here: https://imgur.com/a/XCdir5d Emergency visits with confirmed COVID-19 in Washington state facilities using state's weekly % of Emergency Visits and HHS's summed ED visits by week. Because the HHS's ED visits update the Monday after the state's ED % report, the most recent week of summed visits is the average of the past three weeks of emergency visits due to ALL causes multiplied by the state's most recent ratio of visits being due to COVID-19. Second most recent week of data is the actual count of ED visits due to COVID-19 as reported on Monday.  pre:ED Visits Changes in Recent Counts: 7-Day week of: 4wk ago 3wk ago 2wk ago 1wk ago This week Total: Sep 10 - - - - + 1,420 1,420 Sep 03 - - - 1,362 + 66 1,428 Aug 27 - - - 1,205 + 58 1,263 Aug 20 - - - 1,150 + 0 1,150 Aug 13 - - - 1,009 + 0 1,009 All Older - - - 21,961 + 474 Total new hospital admissions in Washington state facilities with laboratory confirmed COVID-19 as reported by the HHS as summed weekly totals of pediatric and adult admissions. The report I pull this from updates the Friday after the state's update so the most recent week is actually averaged from the past three weeks and should be considered with a grain of salt. Second most recent week of data is the actual count as reported last Friday.  pre:Hosp. Admissions Changes in Recent Counts: 7-Day week of: 4wk ago 3wk ago 2wk ago 1wk ago This week Total: Sep 10 - - - - + 302 302 Sep 03 - - - - + 324 324 Aug 27 - - - 290 + 1 291 Aug 20 - - - 283 + 7 290 Aug 13 - - - 232 - 232 All Older - - - 8,179 + 221  pre:Beds in Use Changes in Recent Counts: 7-Day week of: 4wk ago 3wk ago 2wk ago 1wk ago This week Total: Sep 10 - - - - + 2,268 2,268 Sep 03 - - - 2,401 - 15 2,386 Aug 27 - - - 2,163 + 3 2,166 Aug 20 - - - 1,806 + 2 1,808 Aug 13 - - - 1,715 + 3 1,718 All Older - - - 65,149 + 14 Deaths certificates coded as or referencing COVID-19 in WHALES with a corresponding positive lab (including postmortem) as reported in WDRS. Does not count if the individual only tested positive with a home test before dying, or in cases when there is a positive lab but COVID-19 is not referenced in cause of death.  pre:Deaths Changes in Recent Counts: 7-Day week of: 4wk ago 3wk ago 2wk ago 1wk ago This week Total: Sep 10 - - - - - - Sep 03 - - - - + 11 11 Aug 27 - - - 17 + 16 33 Aug 20 - - - 18 + 1 19 Aug 13 - - - 26 - 26 All Older - - - 955 + 4 Positive clinical cases (tests administered at CLIA certified or CLIA waived labs) as reported by healthcare facility, is not specific to individual's residence. Positive home tests reported to the state have been removed from the official counts.  pre:Cases Changes in state counts reported: 7-Day week of: 4wk ago 3wk ago 2wk ago 1wk ago This week Total: Sep 10 - - - - + 4,059 4,059 Sep 03 - - - 3,663 + 186 3,849 Aug 27 - - - 3,908 + 35 3,943 Aug 20 - - - 3,313 + 5 3,318 Aug 13 - - - 2,817 + 6 2,823 All Older - - - 88,318 + 11 Zantie has issued a correction as of 06:51 on Sep 21, 2023 |
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Sep 21, 2023 01:26
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Teabag Dome Scandal posted:Isn't this what Bieber is dealing with for the last couple of years? We're not allowed to discuss it lol
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Sep 21, 2023 01:26
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StratGoatCom posted:Anyway, lmao if covid just ends making a streak of one term presidencies through end of term hellwaves. Why should any of us give a poo poo how many terms presidents serve?
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Sep 21, 2023 01:27
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Eh, I think Bieber would be okay, IIRC we know he had a long covid diagnosis, but again, we know why it's happening, gawking and guessing is both weird and redundant, it's only interesting if they break omerta.Loucks posted:Why should any of us give a poo poo how many terms presidents serve? because a bunch of presidents all falling into the same trap like Dwarf Fortress Dwarfs is is very stupid and very funny in a very depressing way.
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Sep 21, 2023 01:28
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Would Guillain-Barre fall under the definition of long COVID if the cause was COVID?
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Sep 21, 2023 01:28
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StratGoatCom posted:because a bunch of presidents all falling into the same trap like Dwarf Fortress Dwarfs is is very stupid and very funny in a very depressing way. OK. We just disagree about whether a president dying is depressing in any way. They’ll just install another sociopathic exploitation enthusiast. But get your kicks where you can imo.
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Sep 21, 2023 01:36
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Raskolnikov2089 posted:Would Guillain-Barre fall under the definition of long COVID if the cause was COVID? I’m not sure, but we should probably call it by it’s acronym, GBS
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Sep 21, 2023 01:39
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Loucks posted:OK. We just disagree about whether a president dying is depressing in any way. They’ll just install another sociopathic exploitation enthusiast. But get your kicks where you can imo. Not talking about the president himself, just owning themselves by ignoring covid until it gets so bad it eats his presidency's face by being impossible to ignore. and it happens over and over and over.
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Sep 21, 2023 01:39
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Oh, I get you. I think people will normalize it. Just toss another hundred thousand deaths on the pile. Joe is a one term president though unless they manage to Lock Him Up!
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Sep 21, 2023 01:42
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U-DO Burger posted:I will never ever forgive or forget this haha lmao what the gently caress 
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Sep 21, 2023 01:44
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I do think that if enough powerful people get disabled it will be more and more difficult to normalize, and therefore that is a thing we should all hope happens. The people in such positions I think are mostly psychopaths who are committed to maintaining the power structures even at the risk of their own death or disability though, and that's part of why they expect all of society's underclasses to happily line up for the same (USER WAS PUT ON PROBATION FOR THIS POST)
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Sep 21, 2023 01:44
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Phlag posted:Walgreens does offer PCR tests through a partnership with DOH. I'm not saying that to disagree or prove you wrong - just hoping that some unaware folks are able to snag them. I totally agree that high quality tests should be free and far more easily accessible. None of the Walgreens within an hour drive of me have had them in stock in weeks (at least when I've checked). I called them and they said it wasn’t free and would cost $130 or something. They said insurance wouldn’t cover it either. I honestly don’t know what to believe because info is inconsistent.
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Sep 21, 2023 01:47
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I signed up for that and it said I would get a confirmation email, no email is forthcoming though. Maybe they have to review each application manually?
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Sep 21, 2023 01:48
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Oh my god those upcoming mask mandates in California are only for the staff lmao
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Sep 21, 2023 02:03
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StratGoatCom posted:please don't do this, it's (a) weird, and (b) no need to guess since it was probably loving covid, and thus redundant. plus it's an article about guillan-barre syndrome which, wasn't that explicitly linked to a different viral infection in a study earlier this year? it wasnt covid 
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Sep 21, 2023 02:04
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icantfindaname posted:I do think that if enough powerful people get disabled it will be more and more difficult to normalize, and therefore that is a thing we should all hope happens. The people in such positions I think are mostly psychopaths who are committed to maintaining the power structures even at the risk of their own death or disability though, and that's part of why they expect all of society's underclasses to happily line up for the same I want you to maybe stop and consider whether this utopic vision of a mass disabling event that somehow only affects the powerful and causes them to rationally reorder society to meet their needs is reasonable or actually really loving stupid.
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Sep 21, 2023 02:07
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Teabag Dome Scandal posted:Isn't this what Bieber is dealing with for the last couple of years? Bieber was diagnosed with Ramsey Hunt syndrome
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Sep 21, 2023 02:09
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Fur20 posted:plus it's an article about guillan-barre syndrome which, wasn't that explicitly linked to a different viral infection in a study earlier this year? Mononucleosis.
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Sep 21, 2023 02:09
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U-DO Burger posted:Oh my god those upcoming mask mandates in California are only for the staff lmao Flattening the curve of staff absence.
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Sep 21, 2023 02:10
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Fur20 posted:plus it's an article about guillan-barre syndrome which, wasn't that explicitly linked to a different viral infection in a study earlier this year? There are a whole range of different triggers including COVID-19 that have been implicated in some number of the cases https://en.wikipedia.org/wiki/Guillain%E2%80%93Barr%C3%A9_syndrome#Causes
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Sep 21, 2023 02:11
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eXXon posted:I want you to maybe stop and consider whether this utopic vision of a mass disabling event that somehow only affects the powerful and causes them to rationally reorder society to meet their needs is reasonable or actually really loving stupid. I wonder if chronically ill billionaires would suddenly decide to provide for the chronically ill poor or if, instead, they would grind the poor up into a paste and spread it on themselves in the hopes of a cure Which of these two scenarios seems more likely Hmmm
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Sep 21, 2023 02:13
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a long Covid billionaire would absolutely spend all their money finding a cure and then recoup all their money selling doses to other billionaires. nobody else can afford it
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Sep 21, 2023 02:15
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Fascinating to me how so many of these "harmless" viruses we've let circulate over the years are biting us in the rear end down the road. I'm sure COVID will be different.
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Sep 21, 2023 02:16
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shazbot posted:a long Covid billionaire would absolutely spend all their money finding a cure and then recoup all their money selling doses to other billionaires. nobody else can afford it The cure for long covid is an army of servants to do all the physical and cognitive work required to maintain your body and empire for you like you're Baron Harkkonnen so
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Sep 21, 2023 02:17
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Pingui posted:Flattening the curve of staff absence. lol
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Sep 21, 2023 02:20
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there are plenty of rich and powerful who are hiding their long Covid suffering, but there are also plenty of rich and powerful who have publicly stated that they have long Covid and are miserable and it’s in one ear and out the other with the general public. there is no shortage of articles about them and none of them get much traction except with the Covid conscious crowd, which is not a great ad demographic
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Sep 21, 2023 02:20
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| # ? May 25, 2024 06:42 |
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yoloer420 posted:Are there issues with PCR vs LAMP for covid testing? Is one more precise than the other? or are the reagents cheaper for PCR? I'm just wondering why PCR is used when LAMP seems easier in every possible way. - The ease of running the reaction (temperature-wise) doesn't really matter in a clinical setting. Hospitals and clinical labs already all have PCR instruments that can run lots of samples. Those instruments can generally run LAMP too, but frankly, nobody gives a gently caress. - LAMP is notoriously prone to false positives. PCR is not. In terms of creating a new test from scratch: - Ecosystem. PCR has been around a lot longer, so tons more people are trained in it and know how it works. Major players in this space like Roche, Thermo Fisher, Hologic, etc already have all the manufacturing infrastructure spun up for PCR. This is important since LAMP and PCR use different enzymes and have some other important chemical differences too. Enzymes used in modern PCR are highly, highly engineered too to further improve the reactions. (LAMP has some engineered enzymes as well, but not nearly as many.) - Further, ecosystem-wise, making new PCR and LAMP assays means designing new primers. Those are the short (≈20 bases for PCR, up to 40ish for two of LAMP's characteristic primers) bits of DNA that tell the enzymes where to copy. Figuring out what the primer sequences need to be is a bioinformatics/comp bio problem, and the PCR-related tools here are mature. That is not true for LAMP. (I'd bet that companies like Lucira had to make their own, especially for stuff like Flu, which is not trivial.) And the primer design for LAMP is ***a lot*** harder than for PCR because it has 8 priming sites to PCR's 2 or 3. The sites all need to meet certain characteristics and be in a relatively tight space on the genome, and you don't want the virus to mutate at those sites because it might prevent the primer from binding and then whoopsie, your reaction now has impaired sensitivity or doesn't work at all. This has already happened in PCRs where you get S-gene target failure. LAMP is a lot more vulnerable to it because again, 8 sites instead of 2 or 3, so there's that many more chances for mutations to break your assay. - It's harder to multiplex LAMP reactions. You can run like 3 or 4 PCRs in the same reaction tube if you're using probes with different color reporter dyes. This means you can, say, look for 2 SARS-CoV-2 genes and two controls all at the same time. Or you can do FluA + FluB + RSV + control at the same time. Doing this with LAMP is...a tall order, if it's possible at all, for a lot of reasons. And sensitivity is maybe theoretically similar with super well-designed LAMP reactions, I guess, but there seem to be more top-notch PCRs than top-notch LAMPs. ¯\_(ツ)_/¯
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Sep 21, 2023 02:38
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